Hello,

We use apathy's medium for florescent slides. Works well and lasts for a long time. You can find the recipe here :

http://publish.uwo.ca/~jkiernan/aqmount.htm 

Masoud, we do not know which components you have in your chemical storage cabinet....So we only can help with sources or recipes....

1) https://www.jacksonimmuno.com/technical/products/faq/mounting

2) see attached articles/files

Good luck, best wishes! 

NB: please respect Copyright issues (if you are using any mentioned method in case of a publication: please cite as appropriate) 

Masoud, to be honest:  

naturally you can read all information which already has been posted in Q&A in this Forum ResearchGate, just using the FUNCTION [right to JOBS in the top menu task line ] in the pull down menu (click the down pointing arrow & then choose QUESTIONS and add your search phrase or word, e. g. ANTIFAD  or Antifade or Antifading) resulting in a link to:

 https://www.researchgate.net/search.Search.html?type=question&query=Antifad

(no warranty until editing within some minute....)

OK: the "search word" to be inserted must be  |  Antifading   |

I think you have to go through a lot of steps from best recipe available (from various testing and trials) to validation. Be informed that this also involves knowledge of (Bio-)chemistry. The type of staining; chromogenic or fluorescent method, the fixation used, the detection system employed etc., are all useful considerations.

I give kudos to your interest on this, quite innovative.

Also, you may find this handout useful.

Mounting solution can be purchased commercially or prepared in the laboratory

We use the following custom mixture. To 90 mL glycerol, add 10 mL

of 10X PBS that had been adjusted to pH 9 with 0.5 M Na2CO3. Dissolve n-propyl

gallate in this solution to 5% (w/v) using bath sonication. Store at −80°C in 200-μL

aliquots.Cited by Methods Mol Biol.2010

Recipe for anti-fade mounting medium

- prepare (or purchase) a 10X PBS stock solution

- prepare a stock solution of 20% (w/v) n-propyl gallate (Sigma P3130) in dimethyl formamide or dimethyl sulfoxide (DMSO). (Note: n-propyl gallate does not dissolve well in water-based solutions.)

- thoroughly mix 1 part of 10X PBS with 9 parts of glycerol (ACS grade 99-100% purity) and slowly add 0.1 part 20% n-propyl gallate dropwise with rapid stirring.

- add sodium azide (0.1%) to prevent bacterial growth and DAPI if needed.

What I do:

- in a 50 mL centrifuge tube, I mix 5 mL 10X PBS + 27 mL glycerol + 20 mL water [I dilute the glycerol]

- I prepare 0.2 g n-propyl gallate in 1 mL DMSO (in a 1.5-mL Eppendorf vial) = 20% n-propyl gallate solution

- I mix 500 mL of the 20% n-propyl gallate solution to the glycerol/PBS/water mix. Vortex intensively.

- I add 500 mL of a 10% sodium azide solution (final concentration is 0.1%). Vortex.

- Finally, I add 5 mg DAPI (powder) to the mix and vortex extensively, cover the tube with aluminum foil and keep at -20oC.

When I want to use the solution, I collect a few mL and filter the solution (0.2 mm) immediately prior to use it.

Easy to make and cheap!!!

Christophe P. Ribelayga ,

it would have been not only nice if you - for the sake of being honest and also to tell the whole text for consideration of colleagues in need of - had cited the source you plagiarized (supposing you were not the author of the webtext from jacksonimmuno.com):

cf the whole information to be found @ :

# https://www.jacksonimmuno.com/technical/products/protocols/anti-fade # :

[QUOTE by Copy and Paste]:

" Recipe for Anti-fade Mounting Medium

In our experience n-propyl gallate added to mounting media reduces fading of fluorescence from many different fluorophores during fluorescence microscopy. The following is a simple recipe for making an anti-fade mounting medium containing n-propyl gallate.

• Prepare a 10X PBS stock solution. • Prepare a stock solution of 20%(w/v) n-propyl gallate (Sigma P3130) in dimethyl formamide or dimethyl sulfoxide. (Note: n-propyl gallate does not dissolve well in water-based solutions.) • Thoroughly mix 1 part of 10X PBS with 9 parts of glycerol (ACS grade 99-100% purity) and slowly add 0.1 part 20% n-propyl gallate dropwise with rapid stirring. "

© Jackson ImmunoResearch Inc. All rights reserved. 872 West Baltimore Pike, West Grove, PA, USA 19390. Tel: 1-800-367-5296

(End of Quote by copy and paste)

Personal DISCLAIMER: Not affiliated with the afore-mentioned Company, no financial interest. Just keeping academic practice/usance.

So be it, from Jackson Immunoresearch with modifications. :)