Which is best tool for alignment of large bacterial sequence?

Hi! I need to align big fasta files (bacterial 16S) . I have tried to align them in some web-servers and freeware, but my files are too big to be run in there. I will be very glad if you could...

16 January 2021 1,266 4 View

What is the best studying timeline after injection of B16-F10 melanoma metastases cell line?

I want to inject B16F10 cells into C57BL/6 mice as a melanoma metastases model to study the speed of metastases in different organs. I need advice about the designing of the best timeline for...

14 January 2021 3,704 1 View

No title

I want to simulate a rough surface in Abaqus CAE by transferring nodes in the input file but I have problem in large number of nodes transferring. so I have following steps to pre-process: first...

13 January 2021 8,417 2 View

Is there a formula or standard protocol for steroid hormones treatment in mice or rats?

hi, I'm going to determine the dosage of testosterone in order to inject to rats or mice subcutaneously. I wonder that is there a particular dose or multiple doses for that or not. thanks.

05 January 2021 5,921 2 View

Which type of press is proper and common for making PZT parts?

What kind of press is using to make electroceramic parts, including PZT parts (hydraulic press, pneumatic press, etc.)? Furthermore, what is the reason for using this type of press? And does the...

28 December 2020 1,215 2 View

How to build .cks file (chemkin file) in ANSYS-Forte for hydrogen-diesel dual fuel?

Dear all, I'm trying to simulate the combustion of hydrogen diesel in dual fuel mode in ANSYS-Forte. I have kinetic reaction mechanisms file( .txt) but in order to build the .cks file in the...

28 December 2020 1,570 3 View

Which techniques do you recommend to be combined with a technique in Big Data Analytics?

I am trying to assess or predict the use of one specific technology that might have several impacts on one operational activity. This technology is supposed to improve the efficiency of the...

24 December 2020 7,497 4 View

Why my PCR started to show extra bands and weird results?

Hello guys, I need your help. I used to do PCR for genotyping of my LCK Cre mice. The PCR was working well. You can see in Fig A (Attached picture). No template NT was clean and negative control...

26 November 2020 4,765 3 View

What quantitative method should I use to assess or predict the economic impact of a specific technology on an operational activity like drilling?

I am trying to assess or predict the use of one specific technology that might have several impacts on an operational activity - drilling in the oil industry. This technology is supposed to...

23 November 2020 3,860 4 View

Abaqus Additive Manufacturing modeler plugin?

I want to download Abaqus AM modeller but I couldn't find it. I ask anyone who has this file please inform me. Thank you.

11 November 2020 6,844 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

How to determine solutionizing time for cast Al alloys?

For wrought alloys soaking time at solutionizing temperature is determined by thickness .Do we have such standards for cast Al alloys

23 February 2021 8,839 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View