Doaa Yamani

6 Questions 35 Answers 0 Followers

Questions related from Doaa Yamani

What are the validation timelines for quantative real time PCR and western blotting results?

What are the validation timelines for quantative real time PCR and western blotting results, is it 24 hours for qpcr and 48 hours for western blotting if so! Why then!?

04 November 2023 5,668 2 View

Why I got low cell viability after thawing!?

As recommended by ATCC I freezed my Calu-3 cell line down(-- 80 °C ) in Complete growth medium supplemented with 5% DMSO but I got low cell viability AFTER just two weeks from freezing them...

01 January 1970 8,287 9 View

How to determine the proper time and concenterations for treatment experiments!?

If you are treating some cell line with chemical substances for the first time in your experiment should we look for recommended concentrations and exposure time in previous literature or should...

01 January 1970 6,914 2 View

After How many paasges should I freeze down my stable cells?

- I expanded my A549 stable cells from 12 well plate to 6 well plate should I freeze them down or expand them more in 25cm flask! ? Because I read: Some clones may lose expression after several...

01 January 1970 7,217 14 View

IC 50 ug/ml clculation from log drug concenterations IN Excel sheet!

Hello Good people, I am quite confused I read so many links here and there but still unable to calculate IC50 in ug/ml from log values like what I have in the attached table image!!!

01 January 1970 7,288 2 View

What is the best starting dilution concentrations to work with in stress experiment!?

If I'm working with Cytokines and Growth Factors whose ranges of stock concentrations are in between1,2,5,10,100 ug/ml...How much Nano grams should I start my trials with Stress Experiments in...

01 January 1970 683 0 View