the glycosyl group usually on C3 and C7, when identifying the flavonol by UPLC-Q-TOF-MS, can we judge the position of glycosyl group based on the mass spectra?
Agree with Stjepan Krešimir Kračun
For example, I was once working on identification of quercetin-3-glucoside and quercetin-3-galactoside. Their MS and MS2 are the same (463 --> 300). I was able to chromatographically separate the 2 compounds using a solvent gradient I'd developed. I'm not really sure if they are able to distinguish based on MS spectra.
Hi,
I agree with Dr.Stjepan Krešimir Kračun and Danh C. Vu .
But I may add, you may identify the compounds with additional seperation dimensions based on mass detection. Ion mobility capabilities of the novel TOF instruments are giving unique CCS values for seperation and secure identification of isobaric compounds. If there is proper library regarding the substances that you are looking for, than it might be a little helpfull for positional judging. Since MS/MS fragmentation could add value for evaluation, by different vendors this ion mobility property could be combined with collision units just before the entrance of the drift tube thus you can differentiate the daughter by mass, charge and shape...
yes, you are right, now I read some paper about IMS-Q-TOF, we have to have standards if we want to know the CCS, this is a problem, but however the IMS add a dimension for the identification. İsmail Emir Akyildiz
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