What are the suitable methods for cloning of a human gene responsible for expression of glycosylated protien?

08 March 2023 0 5K Report

For some therapeutic application-based experiments, we need a bulk amt of human glycosylated protein. How could we clone that particular gene and expressed it in a mammalian cell so we get glycosylated protein in good amounts and conditions?

Badges
Science topic

More Nidhi Puranik's questions See All

How can we conduct DFT for solutions or mixtures ?

Can we conduct DFT studies for Ionic Liquids with Amines at various concentrations? If so, how can we proceed with these studies? Additionally, what parameters, besides IR, NMR, and HOMO-LUMO, can...

18 July 2024 319 1 View

How rise in NPAs leads to high efficiency in private sector banks in India?

Please guide me how MPAs has positive and significant relation with efficiency in Indian Private banks while NPAs are negativity and insignificantly related to efficiency in public and foreign...

27 February 2024 6,654 0 View

How employee productivity is insignificantly related to efficiency in private banks while significantly related in public & foreign banks in India?

I want to analyze the relationship between efficiency and income diversification in Indian banks. Employee productivity (Business per employee) is a control variable in regression that is...

25 February 2024 9,389 0 View

Can constant/intercept be insignificant in regression model if all other conditions are satisfied?

13 February 2024 8,194 5 View

Can fractional logistic regression be used in two stage DEA analysis? (In this technical efficiency is my dependent variable, bounded between 0 to 1)?

In most of the studies tobit regression is used but in tobit model my independent variable is not significant. Whether fractional logistic regression is also an appropriate technique to explore...

01 February 2024 4,641 2 View

What is new in Menstrual Health and Hygiene in terms of marketing?

I am researching the taboo behavior around women's menstrual health and hygiene, and exploring ways to shift it towards marketing through better research design.

16 December 2023 2,530 1 View

ELISA for testing a custom made EGFR antibody?

I want to test the efficacy of a custom made EGFR antibody via ELISA. Can anyone suggest me the best way to do it. Thanks Nidhi

03 December 2023 9,003 2 View

How is the expression pattern of Lon Protease 1 (LonP1) of mitochondria during the course of differentiation in mouse embryonic stem cells?

Does the expression of LonP1 decreases with differentiation?

09 October 2023 9,012 0 View

Why grain size decrease after aging? does precipitates and grain both move together in aging process? if yes then what is driving force ?

Basically I want to know why grain boundary becomes small after aging ,as at temperature increases grain size.

17 August 2023 5,744 0 View

Does the shape of the notch affect the fracture test result?

Generally in fracture test pre cracking takes some time which depends on materials but in my case the pre cracking of an Al alloy sample with square notch is completing very soon like in 10...

16 August 2023 6,855 4 View

Is it possible that PNGase F does not cleavage all glycans?

The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...

01 August 2024 3,519 4 View

Is there any software/web server to add carbohydrate chains to a modelled protein?

Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?

01 June 2024 894 1 View

Buffer for PNGase F?

Hello, I want to use a commercial preparation of recombinant PNGase, but I do not have the buffer. What cofactors or buffer conditions does PNGase F require? I will add the enzyme to cell...

08 February 2024 5,715 1 View

How to find out the type of protein glycosylation?

We want to compare the glycosylated and deglycosylated forms of the target protein on a blot. Tell me how to understand what form of glycosylation a particular protein has? I can’t find reagents...

01 February 2024 6,521 4 View

Tags to label differently glycosylated proteins?

Hello there! I have a protein in my project that undergoes glycosylation. There are two main glycosylation products with different molecular weight. However, I do not have the glycosylation...

04 January 2024 1,113 0 View

Anyone has experience working with oligomer protein?

I expressed my protein in Arctic DE3 cells fused with Sumo-tag. I am using 20% Glycerol in the lysate and elution. I seem to have majority of the protein eluting as oligomer. I have 4 di-sulfide...

24 August 2023 7,487 4 View

Importance of MnCl2 mAb glycan profiling and how the pathway work in glycosyl transferase enzyme?

MnCl2 2 to 20mM help in glycosylation profiling buildup. How does this pathway work and what types of chemical reactions happen after the addition of mNcl2 during cell culture process.

17 April 2023 7,731 0 View

Seek help in suggesting a suitable ELISA kit for measuring serum Advanced glycation end products?

An ELISA kit which can measure all target analytes like CML, CEL, precursors of AGE etc

16 April 2023 5,049 4 View

Is there a publication that discusses expression of different glycosylation enzymes in different CHO cell lines?

Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...

12 April 2023 9,492 2 View

Between greenfluorescent protein and amino 3 glycosyl phosphotransferase (neo) which could be better for level of gene expression?

help

12 December 2022 5,904 1 View