MnCl2 2 to 20mM help in glycosylation profiling buildup. How does this pathway work and what types of chemical reactions happen after the addition of mNcl2 during cell culture process.
As a marketing student I am performing research on client's attitude or barriers when they choose or hire architect or interior designer to construct their houses or renovate their home
08 July 2024 1,263 0 View
Procedure: Streptavidin-magnetic bead suspension (50 µL) was transferred into a vial. The beads were washed twice by the binding/washing buffer and then re-suspended in 100µL of the buffer. A...
16 May 2024 893 0 View
How to get research papers with their cites, and what are the keywords you have to put to get context/articles/papers you need?
06 May 2024 8,904 1 View
Hi A paper titled " Intelligent Computing: The Latest Advances, Challenges, and Future" was recommended by my colleague. It has unique visualizations. I am attaching one of them for reference....
29 January 2024 3,880 1 View
We have injected GFP and td-tomato viral tracing injection in the mice brains to see axonal projections, and we are using tertiary butyl alcohol instead of ethanol for the dehydration process as...
09 October 2023 8,654 8 View
Unit conversion for HCN from mg/Kg to mg/Nm3
27 September 2023 1,414 1 View
Hi everyone, I am doing auditory brainstem response measurements using powerlab AD instruments, but I am not getting the responses for lowest dB level as they are too weak to be measured by...
13 August 2023 3,223 0 View
Hello everyone! I am planning an experiment where I would like to check ubiquitin transfer using confocal microscopy; thus, I intend to add a fluorophore tag to the ubiquitin molecule. I read a...
12 August 2023 7,532 1 View
In my observation, during the pandemic in 2020-21 workers from unorganised sectors learned things to survive and adopted creative ways to complete their tasks. However, people also started...
01 August 2023 2,272 0 View
Aprotinin is used as a plasmin inhibitor. 126KUI helps to inhibit plasmin. We have 126 ph. Euro. U/mL concentration of aprotinin with 0.5mL vial. How much will KIU be there in a 0.5mL vial?
14 May 2023 3,348 0 View
The NS1 protein of the tick-borne encephalitis virus potentially has three glycosylation sites. When processing the PNGase F protein on SDS-PAGE, I observe three bands - a fully glycosylated 52...
01 August 2024 3,519 4 View
Previously I have used GlyProt to add carbohydrate chains with my modelled protein, but the server is not working now. Is there any alternative tool for modeling glycosylated protein?
01 June 2024 894 1 View
Hello, I want to use a commercial preparation of recombinant PNGase, but I do not have the buffer. What cofactors or buffer conditions does PNGase F require? I will add the enzyme to cell...
08 February 2024 5,715 1 View
We want to compare the glycosylated and deglycosylated forms of the target protein on a blot. Tell me how to understand what form of glycosylation a particular protein has? I can’t find reagents...
01 February 2024 6,521 4 View
Hello there! I have a protein in my project that undergoes glycosylation. There are two main glycosylation products with different molecular weight. However, I do not have the glycosylation...
04 January 2024 1,113 0 View
I expressed my protein in Arctic DE3 cells fused with Sumo-tag. I am using 20% Glycerol in the lysate and elution. I seem to have majority of the protein eluting as oligomer. I have 4 di-sulfide...
24 August 2023 7,487 4 View
Hello all researchers: I have started working on a hypothetical protein. In silico (SWISS-prot, Hhpred, I-TASSER) analysis shows its homology to following proteins: 1. Hydrolase (peptidase) 2....
26 April 2023 6,021 2 View
An ELISA kit which can measure all target analytes like CML, CEL, precursors of AGE etc
16 April 2023 5,049 4 View
Hi I am kind of looking to see if a comparative expression level analysis of the different Glycosylation enzymes of different CHO cell lines (DG44, DXB-11 and K1)is available in any publication. I...
12 April 2023 9,492 2 View
For some therapeutic application-based experiments, we need a bulk amt of human glycosylated protein. How could we clone that particular gene and expressed it in a mammalian cell so we get...
07 March 2023 4,641 0 View