What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

I am electroplating Ni on a stainless steel. At temperature and time should I anneal so that Ni thermally diffuses into stainless steel?

I am electroplating Ni on a stainless steel for abrasive application. At temperature and time can I anneal so that Ni thermally diffuses into stainless steel? My intention is to improve the...

20 October 2020 7,092 3 View

What are the hot topics in today's situation where an engineer should focus for betterment of society, e.g. Rural development, Solar farming, etc.?

We engineers sometime busy in doing research in countless unpractical, non urgent, not beneficial to society like areas for increasing count of our research articles. But we know an engineer is a...

17 July 2020 1,950 16 View

Relative telomere length calculation with Ct values?

10 May 2020 7,398 2 View

Can any one suggest me what are improvements i can do in clustering of wireless sensor network based on residual energies?

I have already implemented clustering algorithm for WSN(IoT) based on remaining energies of CH's. But i need to improve algorithm for my academic project. Please suggest me the possible...

01 April 2020 3,357 5 View

Could ecological risk indices applied for water samples?

Could Pollution indicators such as degree of contamination index (Cd) and potential ecological risk index (PERI) proposed by Hakanson (1980) also considered for water samples to represent surface...

24 March 2020 7,345 4 View

What should be the average size of amplicon if I am using LBb1.3 (left T-DNA border) and RP (gene specific Reverse primer) against genomic DNA ?

Hello everyone! I am thinking to amplify T-DNA insertion region using LBb1.3 (left T-DNA border) and RP (gene specific Reverse Primer) against the genomic DNA of one of the T-DNA insertion mutant...

11 March 2020 1,239 3 View

Error while compiling WRF - "compile [-j n] wrf compile wrf in run dir (NOTE: no real.exe, ndown.exe, or ideal.exe generated)" Need solution ?

or choose a test case (see README_test_cases for details) : compile [-j n] em_b_wave compile [-j n] em_convrad compile [-j n] em_esmf_exp compile [-j n] em_fire...

06 February 2020 810 8 View

Which software is mostly used in industry for static structural analysis?

Most used CAE software used in industry for static structural analysis.

25 January 2020 6,260 3 View

Kindly explain how to calculate interaction volume from incidence angle in XRD?

26 November 2019 4,411 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

How to immobolise streptavidin to polyacrylamide using EDC/NHS?

Hi! We are aiming to bind streptavidin to polyacrylamide surface. The EDC/NHS method bounced on me as a common method, utilizing the -NH2 of polyacrylamide and -COOH of streptavidin. Is there any...

17 December 2020 7,944 2 View

I have proposal to extract HPA from snails, dos any researcher have protocol of HPA (Helix pomata aglutinin) extraction?

Lectin fro Helix pomatia agglutinin used to detect O-Linked glycosylation

21 October 2020 6,139 3 View

How do I Glycosylate a Protein?

I am in my first year of a biology degree and i'm struggling with some coursework. How would i go about setting up an expression system to create a glycoprotein. I understand how to over express a...

19 March 2020 6,605 4 View

Can I study three partner interactions with Surface Plasmon Resonance?

We are looking at whether a peptide: RNA as a complex can bind to a glycosylated protein. We have established with SPR that the Peptide binds to RNA. Is it possible to study the binding of the...

01 February 2020 5,807 2 View

Quantifying glycosylated proteins on a SDS-PAGE?

What is the best way to approach (unexpected) glycosylated proteins on a western blot (or in general)? I am looking at some potential anti-fibrotic treatments (Tx) using 2D cultured primary human...

01 February 2020 7,303 3 View

Is there any difference between p-glycoprotein and MDR1 protein?

Hi, Can anybody tell me is there any difference between these two proteins? I am doing western blotting for expression analysis of MDR1 protein. So I am using MDR1 monoclonal antibodies. Predicted...

10 November 2019 1,939 7 View

Does anyone have a protocol to purify glycosylated fibronectin from serum samples?

I am trying to purify glycosylated fibronectin from serum samples. Unfortunately, there is no specific antibody against glycosylated fibronectin. So, I want to use lectin-based affinity...

11 September 2019 1,426 3 View

Can anyone advice a good (sensitive and specific) commercial antibody to be used in western blots that detects LAMP2 in mouse extracts?

I would like to use LAMP2 to evaluate glycosylation in mouse tissues.

03 April 2019 246 0 View