Hello,
I want to use a commercial preparation of recombinant PNGase, but I do not have the buffer. What cofactors or buffer conditions does PNGase F require? I will add the enzyme to cell lysate and then run western.
Thanks!
Here is the protocol from New England Biolabs.
file:///Users/adam/Downloads/PNGase%20F,%209PIV483.pdf
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