HI, I am trying to isolate lymphocytes from lamina propria and IEL. i am using miltenyi kit and i got a good population of CD4 T cells but no Foxp3 cells. It is surprising as it was described that we had a good Foxp3 subset . I am using mice that GFP as reporter for Foxp3 expression. I dont know really what happens. could someone help me. Thanks.