I need to dissolve it in water for the preparation of SEM-TB media. I prepared 1M stock solution but it is cloudy not clear. I can not use it in the media preparation as it has to be clean.So what can i do?
First find out the data on solubility of manganese chloride in water. If thie is lower than the requested 1M concentration, then you cannot make a 1M solution. I don't have the data in my private library, but you can certainly find the solubility limit.
Thanks Erik Navara , actually they mentioned it is totally soluble in water , but i dissolved in 0.3N HcL AND I HOPE IT WORKS with competent cells
Hello Dina, thank you for posting this interesting technical question on RG. First of all please make sure which form of manganese(II) chloride you have at hand. This compound exists as anhydrous MnCl2 but also as the dihydrate MnCl2·2H2O and the tetrahydrate MnCl2·4H2O. The most common commercially available form is the tetrahydrate. For the respective solubilities please check the Wikipedia entry at https://en.wikipedia.org/wiki/Manganese(II)_chloride. The solubilities of all three forms in water is so high that you can easily prepare 1M solutions. For example, the solubility of the tetrahydrate salt is given as 2.01 g·cm−3 at room temperature (20 °C), while the molar mass (molecular weight) of this form of the salt is 197.91 g·mol−1. This means that preparing a 1M solution is clearly possible. When you observe that the solution is not clear, this could be due to the presence of small amounts of insoluble manganese(II) carbonate (MnCO3) or manganese(II) hydroxide, Mn(OH)2. The turbidity can easily be removed by adding a few drops of concentrated HCl. Just make sure that the slightly acidic environment is compatible with your SEM-TB media.
Good luck with your experiments and best wishes!
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