Hello everyone!
I am working on my undergraduate thesis. After I used the DNeasy® PowerSoil® Pro Kit to extract DNA from a coral mucus sample I use 2 microliters of DNA to measure the output's concentration with Qubit. Although I am getting high concentrations whenever I run the samples on agarose gel no bands appear and the entire column appears to be degraded. I have been told that this would not hinder my journey towards sequencing these samples (16s rRNA). What am I doing wrong? What can I do? Thank you in advance.
A high dsDNA concentration on Qubit may indicate intact DNA, but if gel electrophoresis shows degradation, it suggests DNA quality issues. Possible causes include shearing during extraction or poor storage. Reevaluate extraction methods and storage conditions to improve DNA integrity for accurate downstream applications.
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