In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How to reduce or increase CFU/ml value ?

I collected the water sample from pond and 1 ml of pond sample diluted with 9ml with water and 100 microliter was cultured into the MacConkey agar plates .Finally,80 colonies were formed in the...

24 January 2021 1,925 3 View

What are the best models of remote sensors that can be used to monitor water quality in fish ponds?

It has become necessary that such sensors be present in fish farms to maintain water quality and control the spread of disease

30 December 2020 8,293 3 View

Which connectivity metric is best to compare changes in habitat over time (PC, PCnum or EC(PC)? Which is best for prioritization of restoration?

I am currently researching a network of species movement between temporary ponds using Least Cost Paths. With Conefor I have calculated the PCnum, EC(PC), and PC (probability of Connectivity, as...

23 November 2020 6,298 3 View

What are the trends and gaps in ecology of temporary ponds?

Is climate change investigation the only way to undertake new findings and innovation in this field?

18 September 2020 8,607 3 View

1. What is the behavior of nanoparticles of gold at cyanide solution surface?

I'm interested about floating nanogold particles on the surface of cyanide solution! What % of nanoparticles is loosed from the pregnant solution ponds during heap leaching?

31 August 2020 1,377 2 View

What are the suitable methods to extract water quality data from Landsat 8 satellite images?

There are various methods/ algorithms used to extract water quality data from satellite images. Most of them are applied for large water bodies. I am looking for a suitable method to extract water...

29 August 2020 5,204 3 View

No title

05 August 2020 8,976 1 View

Offlavor pond fish and usage of bio-organic agent ?

Impressive sharings! Regarding on that matter,somehow people use kind of tamarind whichever in paste or liquid to get rid offlavor on flesh fish,wondering is it live fish can be done that way...

11 April 2020 2,155 3 View

Any mobile App or software to identify Plankton from microscopic photos?

Are there any mobile applications or software to identify plankton from microscopic photos of pond water? This is needed for a participatory training programme.

09 October 2019 4,097 3 View

How quantify relic DNA in water samples?

I need to quantify the amount of relic/free DNA in water samples from a fish pond. I'm looking for a protocol with these method. Does anyone know of any accurate methodology for this...

21 May 2019 5,636 3 View