How to get rid of aggregates in skeletal muscle homogenates?

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?como elimino articulos que no son propios?

eliminoar articulos que son de tu autoria

05 July 2024 2,107 1 View

How to calculate the proportions in grams or milliliters of the compounds for 60 mL of SAPO-11 zeolite precursor gel?

It is required to make a precursor gel for a SAPO-11 Zeolite, the compounds are the following: * As a source of Al: Aluminum Isopropoxide (98%) Formula: C9 H21 O3 Al PM: 204.24 g/mol Density:...

01 July 2024 617 3 View

Dyslexia and Virtual Reality yes or no?

Do you think the use of virtual reality with dyslexia students is good?

23 June 2024 8,437 2 View

Condensation of a Q8 serendipity FE to a Q4 FE with drilling DOFs?

Hello, Can somebody please give me some reference to a paper or book where it is explained how to condensate: * from a Q8 serendipity FE to a Q4 FE with drilling DOFs (16 DOFs to 12 DOFs). *...

05 June 2024 936 1 View

How about for EVT?

Thematic analysis (inductive, deductive, or hybrid?) for Expectancy Violations Theory abt online and offline personas of Female gaming streamers. Which would be best following the framework of...

01 June 2024 4,030 0 View

Inductive or Deductive?

Using thematic analysis on our research on Identity Negotiation of Female Gaming Streamers in our local area, we have developed INT elements (communication process, biculturalism, and...

18 May 2024 9,134 2 View

Is Thematic Analysis applicable for Identity Negotiation Theory?

I've been searching for a specific analysis that falls under INT in the B.A. Communication path. I was wondering if this analysis could be applicable if done on Female Gaming Streamers in a...

18 April 2024 587 1 View

¿Cómo la multiculturalidad afecta a la eficacia de los programas de intervención social y qué enfoques son más exitosos?

La multiculturalidad suele influir en la eficacia de programas de intervención social. Algunos enfoques incorporan la sensibilidad cultural, participación comunitaria y la adaptación para abordar...

11 March 2024 8,028 1 View

¿Cómo la tecnología actual puede estar impactando en la accesibilidad de los servicios sociales y la participación comunitaria?

Debido al avance de las tecnologías, hay varias apps para poder acceder a citas con los servicios sociales, al igual que facilitan el acceso a la información, asistencia y recursos entre otras cosas.

11 March 2024 7,486 3 View

¿Qué causa el abandono escolar entre jóvenes en riesgo de exclusión y cómi pueden los programas sociales reducirlos?

Con esta cuestión se busca identificar los factores de riesgo que provoca el abandono escolar entre los jóvenes en la actualidad que se encuentren en situación de riesgo, como la falta de apoyo...

04 March 2024 3,628 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View