Irais Rivera-Álvarez

3 Questions 7 Answers 0 Followers

Questions related from Irais Rivera-Álvarez

Has anyone quantified DAPI fluorescence by lysing the cells and taking some of the lysate volume to quantify it in a fluorescence microplate reader?

We need to normalize an experiment according to the relative number of cells, and we do not have a fluorescence microscope, so we were thinking of staining the cells nuclei with DAPI, lysate the...

06 November 2018 4,496 6 View

How to get rid of aggregates in skeletal muscle homogenates?

Hello! I used ripa10x to extract skeletal muscle protein for western blot, after a few weeks I observed that my samples had aggregates and I don't know how to get rid of them. Any suggestions?

06 January 2017 5,658 2 View

Can anyone help me with a protocol to isolate gastrocnemius rat muscle fibers?

I am trying to isolate fibers in order to analize mitochondrial membrane potential using confocal microscopy.

06 April 2015 998 3 View