I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test macrophages and neutrophiles against pathogenic APEC strains.
I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.
03 April 2019 7,241 9 View
I am using sRNA to silence a gene in E coli. for expression analysis, I proceed for qPCR. so I grow the wild type and silenced type E. coli and extract RNA without adding any antibiotics except...
03 April 2019 7,313 1 View
I am using phagmids as a silencing technique, so there is need of co-transformation of both phagmid and m13 plasmids for core production and insertion of Phagmid into m13 phage core, to avoid...
02 March 2019 6,160 5 View
I have data of HBV, HAV, Polio and Adeno viruses prevalence among different cities? how can i interpret the results based on Odd ratio, risk factor or Mann whitney test? is there any other...
31 December 2018 7,030 5 View
MLST tool used for screening of housekeeping genes in a particular genome. Is this possible to search for those genes which i want to find out there presence ? I have about 30 genomes of a...
11 December 2018 9,058 4 View
I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...
08 September 2018 7,466 4 View
Hello everyone, I have almost completed my MS thesis degree. i worked on sRNA expression and silencing of virulent gene of avian Pathogenic E coli, hoping to publish my work soon. I want to pursue...
01 January 1970 6,494 6 View
I run qPCR with, Control sample (target gene and HC gene) similarly with Treated sample?( target gene and HC Gene) and use livak methods for fold change, I got the value 0.17923 for 2^-ΔΔCt. my...
01 January 1970 2,557 7 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...
31 July 2024 8,666 3 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View