What type of Hemolysis is this?

I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.

03 April 2019 7,111 9 View

Is Rifampicin addition is necessary for the quantification of total RNA of E coli wild type and Silenced ?

I am using sRNA to silence a gene in E coli. for expression analysis, I proceed for qPCR. so I grow the wild type and silenced type E. coli and extract RNA without adding any antibiotics except...

03 April 2019 7,215 1 View

Can plasmid with f1 ori, insert into m13 phage outside the cell?

I am using phagmids as a silencing technique, so there is need of co-transformation of both phagmid and m13 plasmids for core production and insertion of Phagmid into m13 phage core, to avoid...

02 March 2019 6,070 5 View

Which statistic can be apply on prevalence of HBV, HAV, Polio and Adeno viruses among different cities?

I have data of HBV, HAV, Polio and Adeno viruses prevalence among different cities? how can i interpret the results based on Odd ratio, risk factor or Mann whitney test? is there any other...

31 December 2018 6,937 5 View

How we can use Multi-locus Sequence Typing (MLST) tool for finding our desire genes?

MLST tool used for screening of housekeeping genes in a particular genome. Is this possible to search for those genes which i want to find out there presence ? I have about 30 genomes of a...

11 December 2018 8,929 4 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

08 September 2018 7,364 4 View

Planing PhD in Molecular Microbiology any suggestions?

Hello everyone, I have almost completed my MS thesis degree. i worked on sRNA expression and silencing of virulent gene of avian Pathogenic E coli, hoping to publish my work soon. I want to pursue...

01 January 1970 6,383 6 View

No title

01 January 1970 2,328 7 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View