Sayed Abdullah

9 Questions 13 Answers 0 Followers

Questions related from Sayed Abdullah

Is Rifampicin addition is necessary for the quantification of total RNA of E coli wild type and Silenced ?

I am using sRNA to silence a gene in E coli. for expression analysis, I proceed for qPCR. so I grow the wild type and silenced type E. coli and extract RNA without adding any antibiotics except...

04 April 2019 7,287 1 View

What type of Hemolysis is this?

I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.

04 April 2019 7,192 9 View

Can plasmid with f1 ori, insert into m13 phage outside the cell?

I am using phagmids as a silencing technique, so there is need of co-transformation of both phagmid and m13 plasmids for core production and insertion of Phagmid into m13 phage core, to avoid...

03 March 2019 6,133 5 View

Which statistic can be apply on prevalence of HBV, HAV, Polio and Adeno viruses among different cities?

I have data of HBV, HAV, Polio and Adeno viruses prevalence among different cities? how can i interpret the results based on Odd ratio, risk factor or Mann whitney test? is there any other...

01 January 2019 6,994 5 View

How we can use Multi-locus Sequence Typing (MLST) tool for finding our desire genes?

MLST tool used for screening of housekeeping genes in a particular genome. Is this possible to search for those genes which i want to find out there presence ? I have about 30 genomes of a...

12 December 2018 9,014 4 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

09 September 2018 4,250 0 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

09 September 2018 7,426 4 View

Planing PhD in Molecular Microbiology any suggestions?

Hello everyone, I have almost completed my MS thesis degree. i worked on sRNA expression and silencing of virulent gene of avian Pathogenic E coli, hoping to publish my work soon. I want to pursue...

01 January 1970 6,449 6 View

How to interpret qPCR analysis of 2^-ΔΔCt through livak Method?

I run qPCR with, Control sample (target gene and HC gene) similarly with Treated sample?( target gene and HC Gene) and use livak methods for fold change, I got the value 0.17923 for 2^-ΔΔCt. my...

01 January 1970 2,508 7 View