I run qPCR with, Control sample (target gene and HC gene) similarly with Treated sample?( target gene and HC Gene) and use livak methods for fold change, I got the value 0.17923 for 2^-ΔΔCt. my methods was like
Wild type APEC
ΔCt = Ct(csgA) – Ct(uida)
Silenced APEC
ΔCt = Ct (csgA) –Ct (uida)
ΔΔCt = ΔCt (Silenced) – ΔCt (Wild type)
Relative Expression rate =2–∆∆Ct
now how to interpret what is the fold change how to say its reduction or induction?