What type of Hemolysis is this?

I streaked Avian pathogenic E coli on blood agar plate. i found the results shown in picture, i am not sure what type of hemolysis is this, please guide me.

03 April 2019 7,111 9 View

Is Rifampicin addition is necessary for the quantification of total RNA of E coli wild type and Silenced ?

I am using sRNA to silence a gene in E coli. for expression analysis, I proceed for qPCR. so I grow the wild type and silenced type E. coli and extract RNA without adding any antibiotics except...

03 April 2019 7,215 1 View

Can plasmid with f1 ori, insert into m13 phage outside the cell?

I am using phagmids as a silencing technique, so there is need of co-transformation of both phagmid and m13 plasmids for core production and insertion of Phagmid into m13 phage core, to avoid...

02 March 2019 6,070 5 View

How we can use Multi-locus Sequence Typing (MLST) tool for finding our desire genes?

MLST tool used for screening of housekeeping genes in a particular genome. Is this possible to search for those genes which i want to find out there presence ? I have about 30 genomes of a...

11 December 2018 8,929 4 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

08 September 2018 7,364 4 View

How to Extract hetrophiles and macrophages from Chicken blood?

I need to know an easy protocol of Hetrophiles and Macrophages extraction from blood specially from Avian/chicken. and also want to know some related knowledge which can help me to test...

08 September 2018 4,153 0 View

Planing PhD in Molecular Microbiology any suggestions?

Hello everyone, I have almost completed my MS thesis degree. i worked on sRNA expression and silencing of virulent gene of avian Pathogenic E coli, hoping to publish my work soon. I want to pursue...

01 January 1970 6,383 6 View

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01 January 1970 2,328 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View