I have one PCR workstation.

I performed my RNA extraction/cDNA synthesis on a separate bench (outside hood).

I now have very concentrated cDNA.

In previous labs I had 3 work stations.

1: Prepare primer/probe working stock

2: Prepare serial dilution of concentrated template

3: Preparing master mix and plating serial dilutions

I now have one workstation.

Would you perform you cDNA dilution outside of the workstation?

Or would you do it inside and just turn on UV light/spray DNAzap before plating?

Any advice on PCR workflow would be useful.

Thank you!