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When I work with this method, all proteins are not completely transferred from the gel to the membrane (PVDF), and after staining, there is still some protein remaining on the gel. I'm looking for...
01 February 2018 8,962 6 View
I could not see the protein bands very clearly when painting coomassie Blue.
11 December 2017 8,680 5 View
I want calculate activity enzyme as peroxidase in nanocomposite with the 4-AAP and H2O2 substrates(method Worthigton), but I dont have formula standard. Please guide me. Thanks Sepideh
31 December 2016 6,719 6 View
To measure the peroxidase substrates in the presence of phenol and 4-aminoantipyrine Worthington book value of Epsilon is 6.58 was reported . But I read somewhere else that is not a constant...
09 October 2016 5,495 4 View
Please help me
31 December 2015 6,434 0 View
How to prepare 25mM sodium phosphate buffer with pH 4.0 ?
31 December 2015 3,256 0 View
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03 April 2015 3,921 0 View
Effect of temperature on protein stability. Whether due to decreased absorption due to temperature increase is related to the denatured of protein structure. Please guide me.
01 January 1970 4,541 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
05 August 2024 8,946 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
The delta function seems produce logical contradictions when analyzed on a fundamental level. I would be curious if anyone else agrees.
31 July 2024 10,109 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
