Please help me
When I work with this method, all proteins are not completely transferred from the gel to the membrane (PVDF), and after staining, there is still some protein remaining on the gel. I'm looking for...
01 February 2018 8,962 6 View
I could not see the protein bands very clearly when painting coomassie Blue.
11 December 2017 8,680 5 View
I want calculate activity enzyme as peroxidase in nanocomposite with the 4-AAP and H2O2 substrates(method Worthigton), but I dont have formula standard. Please guide me. Thanks Sepideh
31 December 2016 6,719 6 View
To measure the peroxidase substrates in the presence of phenol and 4-aminoantipyrine Worthington book value of Epsilon is 6.58 was reported . But I read somewhere else that is not a constant...
09 October 2016 5,495 4 View
How to prepare 25mM sodium phosphate buffer with pH 4.0 ?
31 December 2015 3,256 0 View
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10 November 2015 2,521 0 View
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03 April 2015 3,921 0 View
Effect of temperature on protein stability. Whether due to decreased absorption due to temperature increase is related to the denatured of protein structure. Please guide me.
01 January 1970 4,541 2 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?
03 August 2024 4,624 3 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.
31 July 2024 9,140 2 View
Please note it
29 July 2024 4,920 3 View
https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/
29 July 2024 5,161 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
