The factors involved are the coomassie blue dye concentration, period of exposure, period of destaining and most importantly the amount of your protein and weather it is still sound or denatured. My suggestion run more protein.

Dear Savari

Thank you for your guidance.

The concentration of your loaded protein goes a long a way in determining the intensity of your bands

Hi Thomas

The protein concentration problem is not high because of the high concentration of protein loaded.

I do not know what is the problem !!

In fact, with coomassie Blue coloring in comparison the silver nitrate shows a lower concentration of protein.