Sepideh Esmaeily

9 Questions 21 Answers 0 Followers

Questions related from Sepideh Esmaeily

How can in test Western Blottes transfer all proteins from gel to membrane (PVDF)?

When I work with this method, all proteins are not completely transferred from the gel to the membrane (PVDF), and after staining, there is still some protein remaining on the gel. I'm looking for...

02 February 2018 8,934 6 View

How can I get a better coloration of the electrophoresis gel using coomassie Blue method?

I could not see the protein bands very clearly when painting coomassie Blue.

12 December 2017 8,647 5 View

How is the activity of an enzyme-like nanocomposite calculated?

I want calculate activity enzyme as peroxidase in nanocomposite with the 4-AAP and H2O2 substrates(method Worthigton), but I dont have formula standard. Please guide me. Thanks Sepideh

01 January 2017 6,681 6 View

To calculate the number epsilon enzyme activity How do I report?

To measure the peroxidase substrates in the presence of phenol and 4-aminoantipyrine Worthington book value of Epsilon is 6.58 was reported . But I read somewhere else that is not a constant...

10 October 2016 5,466 4 View

How to prepare 25mM sodium phosphate buffer with pH 4.0 ?

01 January 2016 3,210 0 View

How is assay peroxidase gold nanoparticles?

Please help me

01 January 2016 6,403 0 View

How to determine the concentration of nano particles get mixed with protein?

11 November 2015 2,499 0 View

How can coat metalic nanoparticles example whit sulfide?

04 April 2015 3,897 0 View

Why the increase temperatures absorption peak uv-vis protein bovine serum albumin reduced?

Effect of temperature on protein stability. Whether due to decreased absorption due to temperature increase is related to the denatured of protein structure. Please guide me.

01 January 1970 4,502 2 View