How can in test Western Blottes transfer all proteins from gel to membrane (PVDF)?

When I work with this method, all proteins are not completely transferred from the gel to the membrane (PVDF), and after staining, there is still some protein remaining on the gel. I'm looking for...

01 February 2018 8,870 6 View

How can I get a better coloration of the electrophoresis gel using coomassie Blue method?

I could not see the protein bands very clearly when painting coomassie Blue.

11 December 2017 8,592 5 View

How is the activity of an enzyme-like nanocomposite calculated?

31 December 2016 6,620 6 View

To calculate the number epsilon enzyme activity How do I report?

To measure the peroxidase substrates in the presence of phenol and 4-aminoantipyrine Worthington book value of Epsilon is 6.58 was reported . But I read somewhere else that is not a constant...

09 October 2016 5,413 4 View

How is assay peroxidase gold nanoparticles?

Please help me

31 December 2015 6,351 0 View

How to prepare 25mM sodium phosphate buffer with pH 4.0 ?

How to prepare 25mM sodium phosphate buffer with pH 4.0 ?

31 December 2015 3,004 0 View

How to determine the concentration of nano particles get mixed with protein?

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10 November 2015 2,441 0 View

How can coat metalic nanoparticles example whit sulfide?

b

03 April 2015 3,843 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View