What could be the possible reason(s) for low correlation between ELISA and Luminex for the same cytokine measures in human plasma?

I have measures on a cytokine from a regular ELISA and from Luminex platform using human plasma. Although the sample dilution varies between platforms, the extrapolated conc. probably should have...

02 March 2019 704 10 View

Can I inactivate DNases in whole blood before plasma collection?

I need to inactivate DNases before incubating blood sample at 37C for my assays. I need to treat blood and incubate for certain time and at the end collect plasma for ELISA. I do not need...

09 October 2017 1,318 2 View

What are the receptors in our scalp that can sense temperature, either dry heat or heat due to pouring of water? Are they same as skin receptors?

11 December 2014 6,022 4 View

Any inputs on iPSC generation from patient specific EBV-lymphocytes?

I am interested in looking at various aspects of patient specific neuronal lines from an iPSC differentiation angle. The iPSCs will be generated from EBV-lymphocytes of patients. Any tricks or...

11 December 2014 2,725 7 View

How do you isolate a membrane fraction successfully to probe with an antibody in a western blotting experiment?

I use biotinylation kit to isolate various fractions from mammalian cell lysate. How to be sure what I see is membrane fraction. Also I need to see expression of my protein in the membrane...

06 July 2014 2,653 1 View

Can saturation kinetics experiment and radioligand uptake experiment give a different result?

At a particular conc. I am getting different values of uptake rate in two different experiments. One is uptake experiment where 'X' is used in 10uM conc. Another is saturation kinetics from 5-50uM...

08 September 2013 5,832 7 View

Can anyone suggest the best endogenous marker or control for plasma membrane fraction in a western blot? Can it be used as a membrane marker in IF?

I am looking for some marker suggestions for using it in confocal as well as western blotting enriching the membrane. I work with rat glioma cell line as well as HEK293T cell line.

07 August 2013 7,011 11 View

What is the best solvent for Carbamazepine (AED) - DMSO or ethanol?

I want to use AED CBZ for my experiments and treat cells with this drug. I want to know what is the best solvent for it. I face some problems determining the right conc. of the drug.

02 March 2013 5,362 1 View

Any suggestions for RNA isolation kit from 'Human brain tissue' required later for qPCR?

Since these tissues are fat laden, trizol isn't working, hence I would like to stick to a kit.

31 December 2012 8,592 7 View

What precautions should I take when working with Anti-epileptic drugs in cell based assays?

Solubility of drugs, EC50 determination, etc? What parameters or specifications should I consider, while working with signalling mechanisms involving AEDs?

11 December 2012 674 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View