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Questions related from Kalpita R Karan
I have measures on a cytokine from a regular ELISA and from Luminex platform using human plasma. Although the sample dilution varies between platforms, the extrapolated conc. probably should have...
03 March 2019 805 10 View
I need to inactivate DNases before incubating blood sample at 37C for my assays. I need to treat blood and incubate for certain time and at the end collect plasma for ELISA. I do not need...
10 October 2017 1,418 2 View
How does brain sense teh temperature through scalp? Any ruote map? From skin, we have fibres running from skin through spinal cord and to brain reaching somatosensory cortex. Is it same when...
12 December 2014 6,114 4 View
I am interested in looking at various aspects of patient specific neuronal lines from an iPSC differentiation angle. The iPSCs will be generated from EBV-lymphocytes of patients. Any tricks or...
12 December 2014 2,824 7 View
I use biotinylation kit to isolate various fractions from mammalian cell lysate. How to be sure what I see is membrane fraction. Also I need to see expression of my protein in the membrane...
07 July 2014 2,776 1 View
At a particular conc. I am getting different values of uptake rate in two different experiments. One is uptake experiment where 'X' is used in 10uM conc. Another is saturation kinetics from 5-50uM...
09 September 2013 5,961 7 View
I am looking for some marker suggestions for using it in confocal as well as western blotting enriching the membrane. I work with rat glioma cell line as well as HEK293T cell line.
08 August 2013 7,153 11 View
I want to use AED CBZ for my experiments and treat cells with this drug. I want to know what is the best solvent for it. I face some problems determining the right conc. of the drug.
03 March 2013 5,462 1 View
Since these tissues are fat laden, trizol isn't working, hence I would like to stick to a kit.
01 January 2013 8,691 7 View
Generally in illumina paired end sequences we are missing out on het indels and looks like its a common problem. Can anyone suggest something to overcome these and which could be helpful in...
12 December 2012 1,267 0 View
Solubility of drugs, EC50 determination, etc? What parameters or specifications should I consider, while working with signalling mechanisms involving AEDs?
12 December 2012 782 9 View
If one has to look at the variations in Affinity (Km) and uptake rate (V or Vmax) of a receptor-ligand uptake phenomena, and compare it across different doses of the ligand, how will it be...
11 November 2012 1,449 9 View
I would like some inputs regarding using the kind of culture dishes, media or any other requirements to start up live cell imaging to see ligand receptor interaction if any is happening in the...
11 November 2012 9,450 16 View
Can it be subtle even if its not in the favor of their emotion? What methods are being used generally to treat emotional disturbances?
10 October 2012 9,308 16 View
I have been trying to get good quality RNA for qPCR from whole blood. The purity and the yield isn't great for cDNA synthesis. Since these are clinical samples, sometimes buffy coat containing...
10 October 2012 6,704 67 View
Any specifications on gel % or buffer conc.?
10 October 2012 252 10 View
Can temperature (especially high temperature) play a role in this phenomena of neurotransmitter binding, internalization or receptor dynamics?
10 October 2012 8,433 10 View
While I am following the trizol method for protein isolation during RNA isolation from EBV cell lines. I am curious as to what is the best method to obtain proteins simultaneously while processing...
10 October 2012 8,146 15 View
The basic idea being detection using two colors and see colocalization on the membrane. How to see membrane part first without permeabilizing and then intra-cytoplasmic part too with triton may be...
09 September 2012 2,917 11 View
