What could be the possible reason(s) for low correlation between ELISA and Luminex for the same cytokine measures in human plasma?

I have measures on a cytokine from a regular ELISA and from Luminex platform using human plasma. Although the sample dilution varies between platforms, the extrapolated conc. probably should have...

02 March 2019 704 10 View

Can I inactivate DNases in whole blood before plasma collection?

I need to inactivate DNases before incubating blood sample at 37C for my assays. I need to treat blood and incubate for certain time and at the end collect plasma for ELISA. I do not need...

09 October 2017 1,318 2 View

What are the receptors in our scalp that can sense temperature, either dry heat or heat due to pouring of water? Are they same as skin receptors?

11 December 2014 6,022 4 View

Any inputs on iPSC generation from patient specific EBV-lymphocytes?

I am interested in looking at various aspects of patient specific neuronal lines from an iPSC differentiation angle. The iPSCs will be generated from EBV-lymphocytes of patients. Any tricks or...

11 December 2014 2,725 7 View

How do you isolate a membrane fraction successfully to probe with an antibody in a western blotting experiment?

I use biotinylation kit to isolate various fractions from mammalian cell lysate. How to be sure what I see is membrane fraction. Also I need to see expression of my protein in the membrane...

06 July 2014 2,653 1 View

Can saturation kinetics experiment and radioligand uptake experiment give a different result?

At a particular conc. I am getting different values of uptake rate in two different experiments. One is uptake experiment where 'X' is used in 10uM conc. Another is saturation kinetics from 5-50uM...

08 September 2013 5,832 7 View

Can anyone suggest the best endogenous marker or control for plasma membrane fraction in a western blot? Can it be used as a membrane marker in IF?

I am looking for some marker suggestions for using it in confocal as well as western blotting enriching the membrane. I work with rat glioma cell line as well as HEK293T cell line.

07 August 2013 7,011 11 View

Any suggestions for RNA isolation kit from 'Human brain tissue' required later for qPCR?

Since these tissues are fat laden, trizol isn't working, hence I would like to stick to a kit.

31 December 2012 8,592 7 View

What precautions should I take when working with Anti-epileptic drugs in cell based assays?

Solubility of drugs, EC50 determination, etc? What parameters or specifications should I consider, while working with signalling mechanisms involving AEDs?

11 December 2012 674 9 View

How to detect heterozygous Indels in exome dataset in humans?

Generally in illumina paired end sequences we are missing out on het indels and looks like its a common problem. Can anyone suggest something to overcome these and which could be helpful in...

11 December 2012 1,161 0 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View