What could be the possible reason(s) for low correlation between ELISA and Luminex for the same cytokine measures in human plasma?

I have measures on a cytokine from a regular ELISA and from Luminex platform using human plasma. Although the sample dilution varies between platforms, the extrapolated conc. probably should have...

02 March 2019 704 10 View

What are the receptors in our scalp that can sense temperature, either dry heat or heat due to pouring of water? Are they same as skin receptors?

11 December 2014 6,022 4 View

Any inputs on iPSC generation from patient specific EBV-lymphocytes?

I am interested in looking at various aspects of patient specific neuronal lines from an iPSC differentiation angle. The iPSCs will be generated from EBV-lymphocytes of patients. Any tricks or...

11 December 2014 2,725 7 View

How do you isolate a membrane fraction successfully to probe with an antibody in a western blotting experiment?

I use biotinylation kit to isolate various fractions from mammalian cell lysate. How to be sure what I see is membrane fraction. Also I need to see expression of my protein in the membrane...

06 July 2014 2,653 1 View

Can saturation kinetics experiment and radioligand uptake experiment give a different result?

At a particular conc. I am getting different values of uptake rate in two different experiments. One is uptake experiment where 'X' is used in 10uM conc. Another is saturation kinetics from 5-50uM...

08 September 2013 5,832 7 View

Can anyone suggest the best endogenous marker or control for plasma membrane fraction in a western blot? Can it be used as a membrane marker in IF?

I am looking for some marker suggestions for using it in confocal as well as western blotting enriching the membrane. I work with rat glioma cell line as well as HEK293T cell line.

07 August 2013 7,011 11 View

What is the best solvent for Carbamazepine (AED) - DMSO or ethanol?

I want to use AED CBZ for my experiments and treat cells with this drug. I want to know what is the best solvent for it. I face some problems determining the right conc. of the drug.

02 March 2013 5,362 1 View

Any suggestions for RNA isolation kit from 'Human brain tissue' required later for qPCR?

Since these tissues are fat laden, trizol isn't working, hence I would like to stick to a kit.

31 December 2012 8,592 7 View

What precautions should I take when working with Anti-epileptic drugs in cell based assays?

Solubility of drugs, EC50 determination, etc? What parameters or specifications should I consider, while working with signalling mechanisms involving AEDs?

11 December 2012 674 9 View

How to detect heterozygous Indels in exome dataset in humans?

Generally in illumina paired end sequences we are missing out on het indels and looks like its a common problem. Can anyone suggest something to overcome these and which could be helpful in...

11 December 2012 1,161 0 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View