07 July 2015 4 7K Report

Can some body provide protocol to cultivate human PBMC in cell culture ?

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Differences of DNA replication and repair between prokaryotes and eukaryotes?

I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both

09 October 2018 341 2 View

In what condition Caspase 3Cleaved would be positive even before Caspase 8?

I checked the apoptotic effect of a natural molecule on cell line. In my western result I do get over expressed PARP Clvd and caspase 3 positive at 24 hours after treatment. When i checked level...

07 August 2016 8,084 3 View

How to use ImageJ software?

I need to quantify the stained picture using ImageJ please let me know step wise how to make use of it. How can i get the area stained ?

03 April 2016 8,715 3 View

Can Bio Rad Gel doc be used for visualization of western blot?

we do have Gel doc which can be used to see Agarose gel stained with EDBR for DNA  i want to know same set can we use for western blot protein visualization without any other accessories? ( using...

31 December 2015 4,498 4 View

How can i get Image Lab™ Software #1709690?

we need Image Lab™ Software #1709690 for bio rad to visualize chemiluminescence, the original software actually got corrupted and getting from company is quite costly any other way to get it?

31 December 2015 505 3 View

What consideration to be taken for choosing endonucleases?

You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...

10 November 2015 2,406 0 View

Can any body suggest how to solubilize bacterial protein extracted by acetone precipitation?

I am working to extract the whole protein from staphylococcus aureus. the steps include treating cell pallets with lysostaphine followed by  water bath sonication. The collected supernatant is...

03 April 2015 4,608 4 View

Can anybody provide a protocol for protein extraction of staph aureus bacteria?

Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?

01 February 2015 7,605 3 View

Can I store blotting paper after treating with ECL pierce, in case I want to develop it later on with X ray film?

How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).

31 December 2014 9,304 4 View

What concentartion of lysostaphine to be used for Staph aureus lysis

what concentration (ug/ml) of lysostaphine should be used to lyse the staph aureues cell wall.

09 October 2014 8,485 4 View

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