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Questions related from Navin Jj
I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both
10 October 2018 306 2 View
I checked the apoptotic effect of a natural molecule on cell line. In my western result I do get over expressed PARP Clvd and caspase 3 positive at 24 hours after treatment. When i checked level...
08 August 2016 8,053 3 View
I need to quantify the stained picture using ImageJ please let me know step wise how to make use of it. How can i get the area stained ?
04 April 2016 8,655 3 View
we do have Gel doc which can be used to see Agarose gel stained with EDBR for DNA i want to know same set can we use for western blot protein visualization without any other accessories? ( using...
01 January 2016 4,445 4 View
we need Image Lab™ Software #1709690 for bio rad to visualize chemiluminescence, the original software actually got corrupted and getting from company is quite costly any other way to get it?
01 January 2016 466 3 View
You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...
11 November 2015 2,369 0 View
Can some body provide protocol to cultivate human PBMC in cell culture ?
07 July 2015 7,243 4 View
I am working to extract the whole protein from staphylococcus aureus. the steps include treating cell pallets with lysostaphine followed by water bath sonication. The collected supernatant is...
04 April 2015 4,569 4 View
Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?
02 February 2015 7,562 3 View
How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).
01 January 2015 9,258 4 View
I have a recombinant protein interleukin as a powder that I need to run in an SDS PAGE of a required concentration. Before mixing it with loading buffer how should I liquefy to get the required...
10 October 2014 5,300 4 View
what concentration (ug/ml) of lysostaphine should be used to lyse the staph aureues cell wall.
10 October 2014 8,420 4 View
I am in need to measure the interleukin in cell culture before and after treatment of cells. Could anybody tell me detailed procedure by flow cytometer how to do this? My doubts are below---- 1....
08 August 2014 3,200 5 View
I am treating bacteria with my molecule and want to see proteins which are suppress or depress by the molecule in staph auresu bacteria. I would like to know what all techniques could be used for...
08 August 2014 6,626 3 View
I am targeting staph aureus with my molecule to see protein profile in treated and untreated staph i am here with attach my recent 2D gel picture please suggest me how to improve it protein...
08 August 2014 1,262 22 View
I have the custom primary and secondary antibodies-HRP tagged got from market, unquoted ELISA plates and target sample also. Now if want to develop in house ELISA what additional thing do I need...
07 July 2014 8,250 4 View
I need to blot for interleukins which are all less than 50KD what membrane would should be used to blot it appropriately? Thanking you
07 July 2014 3,032 5 View
I am getting enough protrein in SDS PAGe but always 2D gel run is a failure for me I am not getting any spot after run dont know what is the problem.
07 July 2014 2,940 3 View
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04 April 2014 2,479 0 View
I am stimulating a J774 cell line and need to check interleukin's expression please provide me a good protocol. How can I get good extract to evaluate interleukin's levels in cell line?
03 March 2014 2,203 0 View
I need membrane and cytoplasmic protein from gram positive bacteria for 2D gel electro. Please suggest me some good protocol in details
03 March 2014 4,300 0 View
I need to extract membrane and cytoplasmic protein from gram positive bacteria for 2D gel.
02 February 2014 6,533 1 View
Articles invited for special thematic issue Journal: Current pharmaceutical design Web of science indexed Impact 3.0 Details: Check attached file
01 January 1970 5,698 0 View