what concentration (ug/ml) of lysostaphine should be used to lyse the staph aureues cell wall.
100 μg/ml
This cocenteration depended to aim of your expriment. For example we use 13.3μg/ml fo PFGE of S.aureues.
-0.02-0.12% of total bacterial culture in grams for cell wall lysis.
Around 100 ug/mL, depending on the density of the bact suspension
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