Differences of DNA replication and repair between prokaryotes and eukaryotes?

I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both

09 October 2018 198 2 View

In what condition Caspase 3Cleaved would be positive even before Caspase 8?

07 August 2016 7,962 3 View

No title

31 December 2015 373 3 View

Can Bio Rad Gel doc be used for visualization of western blot?

31 December 2015 4,356 4 View

What consideration to be taken for choosing endonucleases?

You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...

10 November 2015 2,275 0 View

How to cultivate human PBMC in culture flask?

Can some body provide protocol to cultivate human PBMC in cell culture ?

06 July 2015 7,093 4 View

Can any body suggest how to solubilize bacterial protein extracted by acetone precipitation?

03 April 2015 4,481 4 View

Can anybody provide a protocol for protein extraction of staph aureus bacteria?

Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?

01 February 2015 7,465 3 View

Can I store blotting paper after treating with ECL pierce, in case I want to develop it later on with X ray film?

How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).

31 December 2014 9,152 4 View

What concentartion of lysostaphine to be used for Staph aureus lysis

what concentration (ug/ml) of lysostaphine should be used to lyse the staph aureues cell wall.

09 October 2014 8,265 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View