How to use ImageJ software?

More Navin Jj's questions See All

Differences of DNA replication and repair between prokaryotes and eukaryotes?

I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both

09 October 2018 341 2 View

In what condition Caspase 3Cleaved would be positive even before Caspase 8?

I checked the apoptotic effect of a natural molecule on cell line. In my western result I do get over expressed PARP Clvd and caspase 3 positive at 24 hours after treatment. When i checked level...

07 August 2016 8,084 3 View

Can Bio Rad Gel doc be used for visualization of western blot?

we do have Gel doc which can be used to see Agarose gel stained with EDBR for DNA i want to know same set can we use for western blot protein visualization without any other accessories? ( using...

31 December 2015 4,498 4 View

How can i get Image Lab™ Software #1709690?

we need Image Lab™ Software #1709690 for bio rad to visualize chemiluminescence, the original software actually got corrupted and getting from company is quite costly any other way to get it?

31 December 2015 505 3 View

What consideration to be taken for choosing endonucleases?

You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...

10 November 2015 2,406 0 View

How to cultivate human PBMC in culture flask?

Can some body provide protocol to cultivate human PBMC in cell culture ?

06 July 2015 7,301 4 View

Can any body suggest how to solubilize bacterial protein extracted by acetone precipitation?

I am working to extract the whole protein from staphylococcus aureus. the steps include treating cell pallets with lysostaphine followed by water bath sonication. The collected supernatant is...

03 April 2015 4,608 4 View

Can anybody provide a protocol for protein extraction of staph aureus bacteria?

Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?

01 February 2015 7,605 3 View

Can I store blotting paper after treating with ECL pierce, in case I want to develop it later on with X ray film?

How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).

31 December 2014 9,304 4 View

What concentartion of lysostaphine to be used for Staph aureus lysis

what concentration (ug/ml) of lysostaphine should be used to lyse the staph aureues cell wall.

09 October 2014 8,485 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

Andrew Burgess

Try this:

http://celldivisionlab.com/2015/08/12/using-imagej-to-measure-cell-fluorescence/

or this

http://celldivisionlab.com/2015/08/13/using-thresholds-to-measure-and-quantify-cells-in-image-j/

Pragna Lakshmi

I followed this. It may help you.

http://lukemiller.org/index.php/2010/11/analyzing-gels-and-western-blots-with-image-j/

Taofeek Akin

Kindly help on how to use image J to measure callus index. Thanks