You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or more restriction endonucleases to cut the plasmid and the chromosome?(various genes on the eukaryotic chromosomes are flanked by various nucleotide sequences) gene A by the way is a responsible for production of a protein that could be economically important if it could be produce in bulk.
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