Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to separate epithelial cells?

Hello everybody, Hope you all are doing fine during this difficult time. I am trying to culture mouse oviductal epithelial cells but, the problem is when I culture them there is a mixture of...

31 October 2020 2,640 4 View

What can you interpret from the estimate of NDVI and LST?

I had estimated the Land surface Temperature and Normalised Difference Vegetation Index, the result shows they have positive relation both increases in lower south part areas and higher in upper...

11 September 2020 6,021 4 View

Can entropy be reversed? In the Sci-Fi movie TENET, the concept is used to reverse time, is it possible theoretically?

Second Law of Thermodynamics states that as usable energy is lost, chaos increases - and that progression towards disorder can never be reversed.

01 September 2020 2,528 37 View

CG relaxation with B3LYP in VASP?

I am trying to CG relax a amorphous GeSe system with B3LYP and single K-point (gamma). Even though I have a 250 atom model, the calculations seem to be extremely slow. Is there a way to speed up...

23 August 2020 8,848 4 View

I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

What has been going on regarding blood transfusion and heart transplants despite Covid-19?

Heart transplant and blood transfusions are very sensitive whether or not there be any pandemics. How do you think is the pandemic affecting these and how could this be managed, like how blood...

28 May 2020 3,283 9 View

How is the outbreak of Covid-19 affecting handling of other diseases and the management of patients with non-covid cases in your country?

Although Covid-19 has been the massive pandemic, there are still other patients with poor health due to other diseases. Emergence of dengue and kalazar has been noted in some parts of our country....

25 May 2020 9,648 31 View

What is your opinion on virtual learning in school?

My question relates to the context of a developing country such as Nepal. 1. People, in this time of COVID-19 lock- down, are showcasing the application of virtual classes in schools and...

21 April 2020 7,173 3 View

Is deproteinization must do step while measuring lactate in blood plasma?

Greetings, I am trying to measure L-lactate in mice blood plasma. For that, Is it absolutely necessary to remove protein form plasma sample before measuring lactate?. How much does this affect the...

09 February 2020 7,752 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View