Puskar Thapa

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Questions related from Puskar Thapa

How to control yeast contamination in plant tissue culture lab ?

I am suffering from this contamination I did everything to avoid contamination like hand washing, using sterile equipment, wearing the lab coat, head net, gloves, before culturing plant sterile...

18 August 2017 4,764 13 View

How often lab is fumigated. And how do we perform fumigation with KMNO4 and formaldehyde solution? Could anyone provide me method?

I am a biotechnologist. And I work in Plant tissue culture industry. I got frequently fungal and bacterial contaminated cultured plate/bottles in incubation. I want to get ride of these kind of...

05 July 2017 2,091 10 View

How to control fungi spores in culture room?

I am doing tissue culture of various plants. However, in this summer season, I am facing heavy fungal contamination even though I have been sterilizing working area with 70% ethanol. So I am...

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