Hi Yanfei

Speedvac will definitely work....the only deciding thing will be whether you have the DNA solution in water or buffer......if it is buffer concentrating in a speedvac will reduce the volume but increase the buffer components and they may interfere in downstream reactions....

bye

You could simply Ethanol precipitate it. Add 1/10th volume of 3M NaAc pH 5.2 and 3 volumes of 96% EthOH, vortex and incubate at -20 for about 20 minutes. Centrifute 20 minutes, wash the pellet with 70% EtOH and then resuspend in 1/10th the original volume. Alternatively you can use silica columns but the yield varies a lot with these columns

Yes, Sander, I understand, but I do not want to go through the ethanol precipitation simply because our DNA is too long (~ 150 kb), so an extra step of precipitation might break some chains. So can you suggest some gentler method? Thanks a lot!

Hi Yanfei

you can use 2-butanol to concentrate the DNA samples without precipitation...see the link

http://www.peds.ufl.edu/divisions/cellular/zolotukhin/PDF/cpmb_2.1A.pdf

bye

quick question. can this be an easy solution? original solution-->cap open, in -20C, until water sublimation. then add less water/solvent. As long as using water rather TE, should this be fine?

Can speed vac work?

Hi Yanfei

Speedvac will definitely work....the only deciding thing will be whether you have the DNA solution in water or buffer......if it is buffer concentrating in a speedvac will reduce the volume but increase the buffer components and they may interfere in downstream reactions....

bye

Try to use speed vac

Try to use lyophilization.

Does anyone know if speed vac or lyophilization will impact downstream methylation studies?

I guess lyophilization will not work as it can damage DNA and loss of DNA could result.

Speedvac is the easiest solution..