I am using Eppendorf safe-lock tubes to store DNA/buffer solutions. I put 0.5 mL of the solution into a 2 mL tube, and stored it at 4 degree C in a fridge (lid is closed tightly,and the tube is kept vertical). After 10 days, I saw some water droplets sticking on the wall of the tube, which means I have a higher concentration for the bulk solution on the bottom of the tube. I do not want to centrifuge the water back to the bottom, because that makes me unconfident with the concentration. Does anyone have similar experience with Eppendorf tubes, and know how to avoid this happening?
That's very common phenomenon. You will see evoparation even at 2 to 8 C. Spin it down, mix the contents properly and use it. We never had any concentration issues after this.
Its hard to expect evaporation from aqueous (buffer) solution stored under refrigeration. However, if you are ok with glass vials then use vials commonly used in HPLC's.
That's very common phenomenon. You will see evoparation even at 2 to 8 C. Spin it down, mix the contents properly and use it. We never had any concentration issues after this.
I think that in addition of what has been suggested above, you should also consider that before you closed the cap of the eppendorf tube it was filled with air that has a percentage of humidity. There is also a condensation.
I agree with Rajesh Medisetty and Abdelhalim Boukaba. spin down and use it.
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