14 Questions 21 Answers 0 Followers
Questions related from Yanfei Li
I am looking for a polymer, and here are some requirements for this polymer: This polymer has to be amorphous (not semicrystalline) at all temperatures. Glass transition temperature: -60 C < Tg
04 April 2013 1,386 26 View
There are several geometries people use on commercial rheometers, such as rotating parallel plates, cone-plate, and Taylor-Couette co-cylinder. If there is an inherent stress gradient in these...
02 February 2013 1,488 10 View
I am using Eppendorf safe-lock tubes to store DNA/buffer solutions. I put 0.5 mL of the solution into a 2 mL tube, and stored it at 4 degree C in a fridge (lid is closed tightly,and the tube is...
12 December 2012 6,194 7 View
I have a sample of long chain DNA (~ 100 kb), and it is a mixture of different isoforms (supercoil, relaxed circular, nicked circular, and linear). But I only want relaxed circular from the...
12 December 2012 6,916 1 View
I want to do some rheology on DNA at theta condition. But I do not know how to achieve the theta condition for DNA solutions. Under different NaCl concentrations, I am thinking to use static light...
11 November 2012 7,909 5 View
I am having some DNA solution of 1 mg/ml, and I want to concentrate the solution to 10 mg/ml?
11 November 2012 8,413 11 View
I am studying the rheology of concentrated DNA solution, and I have some questions on the phase separation of DNA at high concentrations. 1. Does it always phase separate by forming liquid...
11 November 2012 5,978 0 View
I have a regular microscope and I want to study the liquid crystal phase of DNA solution, so I actually need a polarizing microscopy. I wonder what components should I add to my regular...
11 November 2012 6,676 17 View
I am doing DNA electrophoresis. I wonder is there a way to quantify the amount of DNA in each band
10 October 2012 2,006 28 View
I have a DNA/TE buffer solution, the molecular weight of the DNA is 185 kb and this solution has some lipids and protein in it (from lysis of E-coli). The dialysis tubing I used is 300,000 MWCO. I...
10 October 2012 5,929 12 View
and a little linear isoform. I run a gel on it, but I saw a bright sharp band stuck in the wells. Then I put
10 October 2012 4,614 6 View
I am performing a phenol/chloroform extraction of DNA. Unfortunately, I have to equilibrate the pH of the phenol by myself, and mix it with chloroform afterwards. The way I did the pH equilibrium...
10 October 2012 5,381 6 View
I am doing DNA solution rheology, and the DNA is dissolved in a buffer solution. I put this solution is a parallel plate of ARG2 and did some frequency sweep and creep tests, all in the linear...
10 October 2012 5,125 5 View
I have a DNA mixture, the largest of which is 185 kbp. I want to use pulsed field gel electrophoresis to analyze this mixture, but I cannot find a detailed protocol regarding the PFGE setting...
10 October 2012 4,465 5 View
