10 Questions 12 Answers 0 Followers
Questions related from Tommy Zhou
The aim is to preserve the most of RNA structures/base pairing after extraction of RNA from virus. so the question can really be read as: regarding the common RNA extraction methods, what are...
02 February 2018 7,228 2 View
working on an experiment to crosslink viral RNAs with proteins via covalent bonds. what would be an easy/feasible but definite way to detect this conjugates from free proteins and free RNAs? is...
05 May 2017 5,290 3 View
Hi, I try to transfect BHK21 cells with some viral RNAs. the agent I use is to transfect is Lipofectamine MessengerMAX from invitrogen. despite clear and bright fluorescence indicating successful...
01 January 2017 7,570 5 View
1\ small protein is in 5% polyethylene glycol solution 2\ can I use larger volume (like 3-4X) of ethanol or other solvent to precipitate protein from PEG solution? would PEG co-precipitate from...
11 November 2016 8,901 3 View
we are preparing to submit a manuscript in field of plant science. However looking at our current budget we realize we won't be able to afford the common $2000 processing fees charged by most open...
04 April 2016 6,919 19 View
Came across this prestained protein marker and the bands indicates different molecular weights in different buffers. How is that? Are these supposed to be made by proteins of constant molecular...
07 July 2015 4,983 3 View
I recently found a phenomenon that I was working on genetic engineering. the plasmid vector was cut with notI and xbaI, and the insert segment PCR is also flanked by these two sites. but there is...
04 April 2015 7,903 8 View
Hi I am looking for any alternative protocols to purify TMV virions, other than the commonly used PEG precipitation. Two main problems that I have with PEG protocol is that, 1\ any...
11 November 2014 1,839 4 View
I am doing this experiment now: agrobacteria carry a viral vector with a EGFP signal, and I tried to figure out the ratio of cells that are infected (have EGFP).The plant I used is N. benthamiana....
10 October 2013 5,238 1 View
Came across a problem I never had before: when I use transmission electron microscope to visualize some tobacco mosaic virus particles, I can see the rod-shaped particles as indicated by blue...
09 September 2013 7,775 2 View
