How does DNA phase separate?

11 November 2012 0 6K Report

I am studying the rheology of concentrated DNA solution, and I have some questions on the phase separation of DNA at high concentrations.

1. Does it always phase separate by forming liquid crystals?

2. How to detect phase separation? by using cross polarized microscopy?

3. How to suppress phase separation? Because I do not want phase separation. Should I control the pH and ionic strength?

4. If it phase separate at some point, is it possible for it to recover upon dilution?

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