hi there, I did tissue culture projects in varsity days and parameters we were collecting included, 1) percent germination (depending on the type of explant), 2) percent decontaminated (to determine the best way to decontaminate your explant), 3) in the proliferation step you'd need to know the best hormone combinations to use to induce multiple shoot formation and/or rooting thus number of shoots per explant, length of shoots, if there's callus, then number of buds per callus, weight of the callus, 4) in rooting you'd consider number of roots, length of roots, if it is a corm/bulb forming, size, weight of the bulb/corm etc.
Once plants are developed through tissue culture, we should study its morphology, anatomy of leaves, roots, survival rate during hardening and acclimatization, root and shoot ratio, performance of plants after field transfer (growth, flowering and fruiting)
in tissue culture you may have surprises so before starting, put an experimental design with different parameters (several hormonal combinations, explant tyoe, plant genotyps and repeat you experiment 2 or three times ) and note all what occurs during incubation you can than treat easily yor results using statistical softwares
whatever is the finding callogenesis shoot formation rooting ............
Good luck
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