I want to design the experiment for cancer (except breast cancer, colon, prostate) treatment with anti tumor agent Hydroxycinnamaldehyde with molecular mechanisms or pathway.
If your drug could target specific gene, you may find a cell line overexpression this gene at first. Then you find a proper dose for drug treatment using MTT assay in this cell line. When these experiments all right, you may test the expression level of target gene and its related pathways after treated with drug. Finally, read some related papers, you could do a better work. Good luck.
Thank you so much Yuen Gao, actually this drug is already used by my supervisor against every type of cancer cell line, and this drug is not specific to any cell, it acts on mostly every type of cell line.i am in search of new pathway regarding cancer cell line.
if so, I think you may do a microarray experiment first. Then you will find which pathway could change significantly. The drug may influence the modification of those pathway members, such as phosphorylation and acetylation.
Yes Thank you Prof.Doctor Jacques, i already read this paper even my lab publish lots of paper regarding HCA etc..but i am new entry to this lab. But i am in search of some new cancer pathway which should treated with HCA/BCA.Once again thank you so much.