Farhan Haider

17 Questions 34 Answers 0 Followers

Questions related from Farhan Haider

How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

11 November 2019 2,486 3 View

Does any one have Experience of PEI transfection with Gastric cancer cell-lines?

Hello, I am working on gastric cancer cell line, i try to transfected the gastric cell lines (SGC-7901, AGS, BGC823) according to standard protocol, but unfortunately they kill my cells, does...

05 May 2019 1,917 4 View

What concentration of G418 (geneticin) and Puromycin you would recommended for Gastric cancer cell line?

I am using gastric cancer cell line for my research, i used Lipofectamine 2000 for transfection, could any one recommend me the good concentration of G418 and Puromycin? regards

09 September 2018 1,263 5 View

Suggest a relevent mouse model for HepG2 cell line?

suggest a relevent mouse model for HepG2 cell line, i am studying gene expression in tumorgensis. our desire gene show engough results in invitro studies, now i want to know about gene function in...

11 November 2017 4,970 6 View

Why so cell detach from polystyrene plate after 24 hours?

I culture oral cancer cells in polystyrene plate, these cell were okay up to 24 hours, after wound has been created to analysed the migration efficiency, after 48 hours when i check again these...

06 June 2015 7,548 7 View

Can any one suggest me some none-paid journals for Microbiology and Plant sciences?

can any one suggest me some none-paid journals for Microbiology and Plant sciences. No worries if journal don't have impact factor or low impact factor but it should be ISI and valid. Regards

01 January 2015 4,698 26 View

Can anyone help with the problem I have been facing in FaDU cell attachment?

hello, I culture FaDU ( oral cancer cell) for few time but i facing problem with their confluence and attachment, even i used standard procedure for culturing? i use DMEM with 10% FBS and desire...

12 December 2014 6,385 6 View

Why there are no clear bands when I run a Western blot for the GADD45a gene?

I ran a Western blot a few times for the GADD45a gene, but I didn't get clear bands, even when I changed the following conditions: High voltage (100V) to low voltage (80V) Gel concentration from...

10 October 2014 4,825 12 View

How can I design an experiment for cancer treatment through Hydroxycinnamaldehyde?

I want to design the experiment for cancer (except breast cancer, colon, prostate) treatment with anti tumor agent Hydroxycinnamaldehyde with molecular mechanisms or pathway.

05 May 2014 1,177 6 View

Could you please suggest a method for metabolites profiling of plant extract through GC MS?

Could someone please suggest a method for metabolites profiling of plant extract through GCMS (aglient 5975c series)? Also, which part of the column would be suitable for plant extract?

10 October 2013 5,462 5 View

How to collect data from tissue cultured plant?

Any specific parameters?

06 June 2013 460 5 View