17 Questions 34 Answers 0 Followers
Questions related from Farhan Haider
Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...
11 November 2019 2,489 3 View
Hello, I am working on gastric cancer cell line, i try to transfected the gastric cell lines (SGC-7901, AGS, BGC823) according to standard protocol, but unfortunately they kill my cells, does...
05 May 2019 1,920 4 View
I am using gastric cancer cell line for my research, i used Lipofectamine 2000 for transfection, could any one recommend me the good concentration of G418 and Puromycin? regards
09 September 2018 1,268 5 View
suggest a relevent mouse model for HepG2 cell line, i am studying gene expression in tumorgensis. our desire gene show engough results in invitro studies, now i want to know about gene function in...
11 November 2017 4,975 6 View
I culture oral cancer cells in polystyrene plate, these cell were okay up to 24 hours, after wound has been created to analysed the migration efficiency, after 48 hours when i check again these...
06 June 2015 7,551 7 View
can any one suggest me some none-paid journals for Microbiology and Plant sciences. No worries if journal don't have impact factor or low impact factor but it should be ISI and valid. Regards
01 January 2015 4,703 26 View
hello, I culture FaDU ( oral cancer cell) for few time but i facing problem with their confluence and attachment, even i used standard procedure for culturing? i use DMEM with 10% FBS and desire...
12 December 2014 6,391 6 View
I ran a Western blot a few times for the GADD45a gene, but I didn't get clear bands, even when I changed the following conditions: High voltage (100V) to low voltage (80V) Gel concentration from...
10 October 2014 4,830 12 View
I want to design the experiment for cancer (except breast cancer, colon, prostate) treatment with anti tumor agent Hydroxycinnamaldehyde with molecular mechanisms or pathway.
05 May 2014 1,181 6 View
Could someone please suggest a method for metabolites profiling of plant extract through GCMS (aglient 5975c series)? Also, which part of the column would be suitable for plant extract?
10 October 2013 5,467 5 View
Any specific parameters?
06 June 2013 464 5 View
I need standard procedure for the tissue culture of a new plant.
01 January 2013 9,974 6 View
any body who conduct research on biofuel plz help me...Malaysian government giving me scholarship and also give some topic to prepare proposal on them...i have no experience in making of proposal...
08 August 2011 8,166 0 View
08 August 2011 3,104 0 View
06 June 2011 5,515 2 View
06 June 2011 9,947 2 View
06 June 2011 7,578 2 View