How to mine the TCGA Database for RNA sequencing?

Anyone with advice on how to mine data from TCGA Database. I am interested in RNA seq data, i have a gene of interest i to analyze but i cannot seem to locate where to indicate the gene i am...

10 November 2019 2,362 3 View

Does any one have Experience of PEI transfection with Gastric cancer cell-lines?

Hello, I am working on gastric cancer cell line, i try to transfected the gastric cell lines (SGC-7901, AGS, BGC823) according to standard protocol, but unfortunately they kill my cells, does...

04 May 2019 1,816 4 View

What concentration of G418 (geneticin) and Puromycin you would recommended for Gastric cancer cell line?

I am using gastric cancer cell line for my research, i used Lipofectamine 2000 for transfection, could any one recommend me the good concentration of G418 and Puromycin? regards

08 September 2018 1,107 5 View

Why so cell detach from polystyrene plate after 24 hours?

I culture oral cancer cells in polystyrene plate, these cell were okay up to 24 hours, after wound has been created to analysed the migration efficiency, after 48 hours when i check again these...

05 June 2015 7,416 7 View

Can any one suggest me some none-paid journals for Microbiology and Plant sciences?

can any one suggest me some none-paid journals for Microbiology and Plant sciences. No worries if journal don't have impact factor or low impact factor but it should be ISI and valid. Regards

31 December 2014 4,427 26 View

Can anyone help with the problem I have been facing in FaDU cell attachment?

hello, I culture FaDU ( oral cancer cell) for few time but i facing problem with their confluence and attachment, even i used standard procedure for culturing? i use DMEM with 10% FBS and desire...

11 December 2014 6,286 6 View

Why there are no clear bands when I run a Western blot for the GADD45a gene?

09 October 2014 4,654 12 View

How can I design an experiment for cancer treatment through Hydroxycinnamaldehyde?

I want to design the experiment for cancer (except breast cancer, colon, prostate) treatment with anti tumor agent Hydroxycinnamaldehyde with molecular mechanisms or pathway.

04 May 2014 1,080 6 View

Could you please suggest a method for metabolites profiling of plant extract through GC MS?

Could someone please suggest a method for metabolites profiling of plant extract through GCMS (aglient 5975c series)? Also, which part of the column would be suitable for plant extract?

09 October 2013 5,316 5 View

How to collect data from tissue cultured plant?

Any specific parameters?

05 June 2013 360 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View