Hello

I have been trying to solubilize oleic acid to add it in cell culture, as done in this article

Article Insulin prevents fatty acid induced increase of adipocyte size

"Oleic acid was prepared as a stock solution 400 µM complexed to lipid-free bovine serum albumin (BSA, Sigma Aldrich) 10% by incubation at 42°C during 2 hours then filtered and stored at −20°C until use. At time of experiments it was diluted at 10 µM in 1% BSA or human lipid-free albumin for human cells (Sigma Aldrich), in either low or high glucose medias (LG, 1 g/L and HG, 4.5 g/L, respectively) with or without FCS (10%) then incubated at 42°C during 2 hours."

I have noticed two things. First, despite the addition of lipid-free BSA and heating, it clogs the filter. So, instead, I tried to solubilize it in DMSO (0,5gOA/mlDMSO) and filter it, which seems to work. However, I still need to adjust its pH after adding it to the media, so I would need to filter it again, putting me back at the same problem.

Second, in the stove, at 37 degrees C, some of the "butter" of the oleic acid accumulates at the bottom, another hint it is not solubilized.

Is there any step I am missing?