How much plasma we need for exsosome isolation for miRNA and LC-MS/MS work ?

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Dominik Buschmann

The answer to this question very much depends on the specific isolation protocol used in the experiments.

I can't comment on the LC-MS/MS part, but for miRNA characterization (small RNA-Seq), we successfully isolated EVs from as little as 1 ml serum by UC.

Eman Thabet

1ml if you are doing NGS for validation

300-500 ul if you are doing rt-qPCR .

According to the

WEBINAR: Exosome miRNA sequencing for biomarker profiling using the Ion S5 System and Ion Total RNA-seq kit

SPONSORED BY: Thermo Fisher Scientific Ion Torrent

Aman Sharma

Even 50 micro liter is good to start with if your isolation efficiency is good.

Drop me you email id. Will send you.