Dhananjay KUMAR

5 Questions 18 Answers 0 Followers

Questions related from Dhananjay KUMAR

How much plasma we need for exsosome isolation for miRNA and LC-MS/MS work ?

I am curious to know much plasma i need for exosome isolation by ultracentrifugation method for miRNA and LC-MS/MS work, is it 2 ml plasma is okay for this work ? thank you in Advance

11 November 2017 2,906 3 View

How to characterize the cell line ?

I have Isolates Hepatic Stellates cells from human tissue. So many articles say that these cells (HSC) do not divide in culture condition. In my culture plates these cell are 2 months old...

03 March 2013 1,599 2 View

Separation of ESD-3 hESCs(mouse embryonic stem cell line) cell from mouse embryonic fibroblast.

Hello ever one mouse embryonic stem cells they are usual grown in feeder layer.And these fibroblast is treated with mitomycine for inhibition of division of this feeder layer. My problem is that I...

01 January 2012 418 2 View

Derbies during HepG 2 2 1 5 cell culture.

Hi everyone i am culturing Hep G 2215 hepatomoma cell lines.My problem is that after 2 -3 day of culture.Small dot dot like stricture seen in flask( But they have not moving like Bactria or they...

01 January 2012 8,250 8 View

Isolation of fibroblast from mouse tail.

Hello every one I need to grow fibroblasts from the mice tail, has anyone done that with the tissue from the mouse tail ? Where can I find protocols for preparing them from the tissues. Please...

01 January 2012 311 2 View