we are planning to do sperm DNA fragmentation tests for infertile patients and we can do by SCD and SCSA. interestingly the results varies. if someone has any experience or idea about it please share.
Dear Syedwaseem Andrabi
The COMET and Sperm Chromatin Dispersion (SCD; HALO) tests were introduced as light microscope tests that don’t require a flow cytometer. Since these tests measure only 50–200 sperm per sample, they suffer from the lack of the statistical robustness of flow cytometric(5000 sperm) measurements. Only the SCSA test has an exact standardization of a fixed protocol. The many variations of the other tests make it very difficult to compare data and thresholds for risk of male factor infertility.
Regards
Sanketh D.S
@sanketh dhumal
Ist of all thanks.
I understand the procedure and standardization but I was curious about the validation. as doing from kit and by flow Cytometry the results vary a lot so don’t understood what should be threshold levels in both cases.
Thanks and regards
Waseem Andrabi
Hello,
If you have the option of doing SCSA, then probably it should be your first choice.
Here are two reviews discussing the sperm DNA assessments:
Article The Sperm Chromatin Structure Assay (SCSA®) and other sperm ...
Article A systematic review on sperm DNA fragmentation in male facto...
Among other things, they also talk about agreement between different methods, including SCSA and SCD.
Regards
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