Is there any way to remove microbial contaminations from the raw semen sample which was not kept in cold chain?

More Syedwaseem Andrabi's questions See All

What is the procedure of using age data in FiSAT II Software? How the data needs to be aligned?

As length data is used to estimate different population dynamics parameters, I want to know what parameters are obtained using the age data and how the data of age of the fish needs to be framed...

28 September 2021 9,391 3 View

What is the main reasons of degeneration after ICSI in human oocytes?

more often we found degeneration in oocytes after ICSI ? was just curious to know what can be the reasons apart from technical error?

23 January 2019 2,350 3 View

How much percentage variation range results in sperm DNA fragmentation, if we do manually by using a kit (SCD) and by flow cytometry (SCSA)?

we are planning to do sperm DNA fragmentation tests for infertile patients and we can do by SCD and SCSA. interestingly the results varies. if someone has any experience or idea about it please share.

11 March 2018 6,726 3 View

Which technique you feel is best for estimationg efforts for web applicaiton development?

which you feel out to be the most poplar effort estimation technique for estimating the efforts required for the development of web software applications. please support you response and kindly...

04 January 2016 1,723 3 View

Onset potential of oxygen evolution in LSV using KOH as electrolyte and Ag/AgCl as reference electrode?

Onset potential of oxygen evolution in LSV using KOH as electrolyte and Ag/AgCl as reference electrode.

07 October 2015 2,769 4 View

What is the applicability of Analogy Based estimation technique in todays software cost estimation world?

To what extent this type of methodology is still in practice and what about its standing in comparison to other methods presently being deployed for cost estimation in software engineering.

02 April 2015 344 6 View

To what extent can one expect there to be change of effort estimation techniques if the mode of development is global?

If there is any software effort estimation technique for general development. if we are going to estimate the efforts when the mode of development is global. to what extent there can be impacts...

20 March 2015 4,243 2 View

What is the best guideline to prepare SRS document?

SRS is Software Requirement Specification

04 February 2015 3,618 5 View

What is Usability in web engineering?

Usability can be defined in Laymen Language as the technique which we can use to check whether all the flows are working as per the per-specified pattern or not? Please can u describe this as per...

03 February 2015 3,991 4 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,366 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

I accidentally removed 5 publications a few minutes ago. How can I add them again to my profile?

23 July 2024 9,145 2 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

Raúl Lagos-Cabré

Hi, again

Taken sperm sample is really easy so take another one!!

Syedwaseem Andrabi

yes But we have to analyse the old one too. Question is not of taking another sample.

Then you lost that sample. If it was kept out of the fridge over night your results won't be right. You won't be able to compare that with other samples, even if you use antibiotics, because there are not the same conditions.