I have prepare dilution buffer and incubation buffer, and I wanted to know how long can i store them in the fridge before the next use
It can probably be stored indefinitely if it's sterile-filtered or if you add a preservative to prevent growth of bacteria and mold.
I would only store it for a week, adding other things might compromise your protocol or give non-specific bands. Fresh is always best!
If sterile-filtered, will that be fine? to avoid preparing it over and over again?
It's not very difficult to make, but in any case I think if you sterile filter it and keep it cold and dark, it should last for a long time. If you see any precipitation in the solution, throw it away.
Dear Godfrey
The PBS buffer even if you sterilizes it changes and for the best results and to calibrate your experiments PBS must be freshly prepared, sterilize and not kept for more than five days. especially when used in biological experiments.
It is so easy to prepare when you use volumetric flasks and an accurate chemical balance to weight the chemicals
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