Kellie Bowen

5 Questions 41 Answers 0 Followers

Questions related from Kellie Bowen

Can anyone point me to a reliable NCAM antibody to use on rat tissues for IHC?

I'm willing to do fluorescence or DAB. Rat muscle, snap frozen, 10um cryostat sections.

11 November 2016 6,805 3 View

Has anyone ever done a protein filtration assay?

I'm looking for alpha synuclein aggregates. I have a few questions about the technique.

04 April 2016 5,925 9 View

Can anyone help with tissue harvest for westerns, specifically kidneys?

I generally flush the rat with saline before harvesting tissues for western blots. I have heard that the blood and other components can interfere with blots. Does anyone else flush the animal...

08 August 2014 7,291 6 View

Does anyone have a suggestion for a loading control for an insoluble fraction?

Insoluble pellet of mouse kidney.

04 April 2014 6,190 4 View

Does anyone know who sells a good Fibronectin antibody that works on mouse tissues?

Also a good protocol for IHC with it.

04 April 2014 1,585 5 View